Rollo Posted on 12:25 pm

Multiplex Fluorescence Western Blot Imaging Systems and Detection Kits

Using chemiluminescence or western fluorescence, the researchers wanted to find proteins of interest in the first experiment. There are many variables involved in performing a Western blot, i.e. use of nitrocellulose or PVDF membranes, use of homemade or commercially available inhibitors, determination of primary and secondary antibody concentrations or selection of suitable detection reagents. 

Optimizing each variable can greatly increase the detection sensitivity for certain Western blot workflows. What's new to recognize the protein of interest in the first try?

Researchers turned to the highly sensitive Western Blot multiplex imaging system to simplify the transition from traditional film-based workflows to desktop imaging. There are companies that provide elisa testing services via https://www.bosterbio.com/services/assay-services/elisa-testing-service.

Image Source: Google

The next-generation CCD camera system combines the advantages of chemiluminescence, UV fluorescence, and colourimetric imaging with the ability to display multi-coloured fluorescent dyes (such as Cy5, Cy3, and Cy2) in quantitative western imaging systems – no film required, no chemicals, and a dark room.

Multiplex fluorescence imaging with its wider dynamic and linear ranges (when combined with the latest Western Blot multicolour kit) allows multiple proteins to be detected in one assay, and the overlapping proteins with the co-migration pattern can be revealed immediately. It also reduces the need to remove and re-check stains for further detection of other proteins, minimizes protein loss and leads to higher productivity and data integrity.

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